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Many of our proposed solutions require the use of multiple DNA markers to accommodate the genetic background of the individuals sampled as cases and controls.
When lakes were sampled multiple times, data from all studies were integrated towards assessing DU status (e.g. multiple DNA markers in phylogenetic studies).
The monophyly of subfamily Thalictroideae has been confirmed based on phylogenetic analyses of multiple DNA markers: rbcL, matK, trnL-F spacer, and 26S nrDNA [ 23], 26S nrDNA [ 24], and atpB, rbcL, and 18S nrDNA [ 39].
By amplifying multiple DNA markers (the trnL intron, internal transcribed spacer region 1 [ITS1], and internal transcribed spacer region 2 [ITsimultaneouslyously, they streamlined the PCR process and increased species resolution for Poaceae, Asteraceae, Cyperaceae, and Rosaceae.
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As has been described in sporadic AD cases [4], neurons of the AD twin exhibited dramatic decrements across multiple DNA methylation markers compared to the non-AD twin.
Having confirmed a strong association between γH2AX puncta and multiple DNA damage markers in these tendon fibroblasts, we then investigated the link with replication status.
The localization of the positive clones selected with each marker at two or more contigs could be multiple copies of the DNA markers in the genome or contig assembly errors [32].
Together, two nuclear DNA, multiple mtDNA, and two plasmid DNA markers were analyzed for real-time PCR amplification using the MyiQ real-time PCR system as well as the SYBR Green I intercalation dye [ 34].
In order to validate the use of AFLP for this study we first determined the experimental reproducibility of the markers generated from multiple DNA samples prepared from a single C. savignyi.
Thus, some DNA markers may identify multiple loci on the physical map, only one of which may have been genetically mapped, leading to contigs being assigned to multiple genetic locations.
Known and as yet undetected QTLs are a valuable method to identify DNA markers, often in multiple genome positions, that can be used to select for improved feedstock varieties before a crop development cycle is complete.
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