G2 arrest involves multiple discrete steps including initiation, maintenance, adaptation and release that occur through distinct mechanisms.
Thus, Lrrk might function as a general regulator of multiple distinct membrane transport steps.
Proteins are degraded by the ubiquitin proteasome pathway via two distinct steps: the covalent attachment of multiple monomers of ubiquitin molecules to a protein substrate and degradation of the tagged protein by the 26S proteasome [ 8, 9].
In this study, we demonstrate that a single human miRNA, miR-124, can concomitantly repress multiple pro-metastatic targets and thereby to inhibit several distinct steps of the invasion-metastasis cascade in breast cancer cells.
In many instances, the novel trait results from multiple genes, which are responsible for different morphological alterations, distinct steps in a novel biochemical cascade, or a combination of both.
The present work demonstrates that miRNAs can also influence later steps of metastasis and that an individual miRNA can intervene at multiple distinct stages of the invasion-metastasis cascade.
For example, Soroka et al. [ 49] revealed that Hsp90 is phosphorylated at multiple sites in the middle and C-terminal domains, which permits regulation of the conformational cycle at distinct steps.
As a final step, we resolve the conflicts among the tandem duplications, which are caused by multiple distinct tandem duplications having overlapping boundaries, by applying an algorithm that relies on the maximum parsimony principle.
In step 3, the HMMER software was used to build and calibrate Hidden Markov Models (HMMs) from multiple, distinct conserved regions, i.e. potential domains, of the MSA.
