Addition of Bcl-2 inhibitors, antimycin or HA14-1, caused reduction of the bioluminescence signals (Figure 6C), demonstrating that three fragments of McLuc1, N-terminal ELuc and FLuc are useful for detecting interactions of a protein associated with multiple distinct protein partners.
However, the question of whether individual channels may contain multiple distinct KCNE proteins in vivo has never been examined prior to this study.
Moreover, the presence of multiple distinct SUMO proteins within cells and the potential for some of these forms to generate polySUMO conjugates [18] raises the possibility that a "SUMO" code exists.
The interaction between multiple, distinct bait proteins on the bait-PARCs with the prey is monitored in living cells using the co-localization of fluorescence signals within an exponentially decaying evanescent field of 50 300 nm depth using total internal reflection fluorescence microscopy (TIRFM).
Multiple, functionally distinct proteins are seen dramatically altered in their expression in the resistant cell line.
Other replication proteins, including PCNA and RPA34, which had been shown to interact with Tim in other studies [45], were not found in our Tim-associated complex, suggesting that Tim may form multiple and distinct protein complexes (Fig. 1C).
The Trp63 gene encoding p63 gives rise to multiple functionally distinct protein isoforms, including TAp63, which encodes for an amino terminal transactivation domain (TA) and ΔNp63, which lacks the N-terminal transactivation domain (ΔN), and is synthesized from an internal promoter [21].
To widen the catalytic repertoire, ancestrally reconstructed sequences from multiple, mechanistically distinct protein superfamilies could be pooled to generate a more complex library.
For example, in the case of α-synuclein, an intrinsically unstructured, highly amyloidogenic, 14.5 kDa protein associated with Parkinson's disease, multiple distinct conformations of the protein monomer have been identified using ESI MS [53,54].
Mitf gene exhibits a split promoter design, which allows it to generate multiple structurally and biologically distinct proteins (Hershey and Fisher 2005).
