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Multiple distinct miRNAs from a single precursor were previously described in plants [ 46, 47].
Individual miRNAs can modulate multiple mRNA targets, and individual mRNAs can be regulated by multiple, distinct miRNAs [ 20].
Cx. quinquefasciatus and Aedes miR-210, miR-252, and miR-2951 arexampleses of multiple, distinct miRNAs arising from one arm of a single hairpin.
In addition to being regulated by multiple members of a miRNA family, an mRNA can be cotargeted by multiple distinct miRNAs families, if it contains the corresponding distinct seed-complementary sites.
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If these mirs are co-expressed, they may target different mRNAs or might interact with the same target transcripts via multiple, distinct miRNA binding sites.
The present work demonstrates that miRNAs can also influence later steps of metastasis and that an individual miRNA can intervene at multiple distinct stages of the invasion-metastasis cascade.
The miRNAs families MIR-Seq01, MIR-Seq03, MIR-Seq06, MIR-Seq07, MIR-Seq08, MIR-Seq12 and MIR-Seq13 had multiple distinct targets.
In this study, miRNA-seq analysis revealed substantial Shh-dependent expression of distinct miRNAs, including miR-342-3p miR-342-3p miR-342-3p
In mouse cells infected by HSV-1, LAT functions as a primary miRNA precursor that encodes four distinct miRNAs [12].
With multiple sites under study, these approaches might result in different or multiple distinct groups.
Based on above analysis, we identified 84 distinct miRNAs, including 19 novel miRNAs.
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