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We further quantified the lifetimes of bonds subject to a constant force, and thus identified multiple dissociation events with rates that agree quantitatively with the force spectroscopy data.
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The evidence for multiple association and dissociation events on the AuNP surface was intriguing, and our global examination of the adsorption of the protein over time demonstrated the truly dynamic nature of protein NP interaction and complex formation.
Conceptually, one could consider p38-mediated phosphorylation or activation as the process by which p38 comes into contact with one of its substrates and performs the necessary modifications, either via one single-step reaction, or multiple dissociation and rebinding events.
(B ) Single molecule time traces showing donor (green) and acceptor (red) intensities exhibits rapid FRET fluctuations with multiple binding and dissociation events within a single time trace (top panel).
Single molecule time traces show multiple dsDNA binding and dissociation events to the same filament because the observed interactions are transient without forming a stable product.
Occasional larger displacements in the Lis1 traces may correspond to "bursts" of multiple rapid steps at the rate observed for free dynein in 1 mM ATP and could reflect Lis1 dissociation events.
The other two categories are disjoint subsets of the inclusive sample that are either enhanced or depleted in single diffractive dissociation events.
If this reflects spontaneous dissociation of AIP1, 10∼20% of AIP1 dissociation events may lead to the formation of free barbed ends.
The corona is dynamic in nature and its composition varies over time due to continuous protein association and dissociation events.
'No amplification' denotes multiple dissociation peaks i.e. non-specific amplification.
(E ) Calculation of the time delay of probe dissociation with respect to RNAP run-off (ΔT off ) from heat maps of all RNAP-DNA and probe-DNA dissociation events post-synchronized by RNAP dissociation (T off ).
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