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In multiple digestion protocols, the order in which releasing enzymes are applied affects the partitioning of fragments into pools.
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DNA was digested using standard digestion protocols for restriction enzymes.
Several in vitro digestion protocols have been described, with a wide range of experimental conditions but no optimised protocol exists.
Sequential digestion protocols may thus reduce the total PCR effort.
Methylation of the MLH1 promoter regions was examined using previously described digestion protocols with some modifications.
Since the presence of labeled whole cardiomyocytes or cardiomyocyte remnants in the isolated cell preparations could confound our results, we took extensive care to ensure efficient cardiomyocyte depletion, by combining a harsh enzymatic digestion protocol with multiple filtering steps.
With a double digestion protocol, the probability of truly obtaining the same fragment multiple times is much higher because the DNA is cut at fixed locations (as opposed to randomly via sonication).
Three multidomain protein systems (calmodulin, a Fyn construct, and transferrin) were used to validate the SUPREX-protease digestion protocol.
Balascio and colleagues succeeded in extracting (using the acid digestion protocol) and subsequently geochemically fingerprinting tephra from two strata.
In-gel digestion was performed according to UCSF in-gel digestion protocol (http://donatello.ucsf.edu/ingel.html; accessed April 27, 2010).
The enzyme-to-protein ratio used in the digestion protocol was 1∶35.
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