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Taken together, these experiments show: (1) that Sp1 −/− cells are able to progress through multiple differentiation steps but are unable to complete terminal differentiation from myeloid progenitors; and (2) that the effect of Sp1 deficiency is cumulative rather than progenitor type specific.
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Instead, typical time scale of macroscopic differentiation of multiple days suggests that some delays between subsequent cell differentiation steps need to be accounted for.
Nevertheless, these pathologic cells may share differentiation steps with osteoblasts at the molecular level.
Furthermore, we conservatively filtered the predictions for expression in multiple cell lines in multiple differentiation stages.
The results showed that these hSKPs had multiple differentiation potentials.
The osteogenic- and adipogenic-induced differentiation experiments confirmed that the obtained ADSCs possessed multiple differentiation abilities.
Human BM-MSCs were trans-differentiated into hepatocyte by using two steps protocol: differentiation step followed by maturation step.
But the signals that control this fundamental differentiation step remain unclear.
Multiple steps of cellular differentiation and the growing complexity of the vasculature formation process were observed in tissue samples harvested at only two different time points (5 and 21 days after implantation) due to local variability resulting from lack of synchronization in vivo.
The secreted signal retinoic acid (RA) has been shown to control multiple, sequential steps of motor neuron differentiation in the ventral spinal cord, including initial patterning of progenitors cells and specification of motor neuron subtype identity [11].
Bone morphogenetic proteins (BMPs) are members of the transforming growth factor-β (TGF-β) superfamily and are involved in the control of multiple key steps of embryonic development and differentiation [6] [8].
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