In mammalian cells, ROS-specific responses such as those regulated by p53, activator protein (AP -1, nucleAP -1ctor (nuclear c-Myc, factorNF -κBer factors caNF -κBeen as part of long-term differentiation programmes that integrate ROS protection and multiple metabolic/adaptative responses.
On the other hand, recent advances in genomics have revealed that cellular differentiation is not governed by a few dedicated transcription factors, but depends on the activities of multiple transcription factors, almost all of which are expressed and used for other differentiation programmes [ 21].
Furthermore, we conservatively filtered the predictions for expression in multiple cell lines in multiple differentiation stages.
Whether differentiation programmes leading to MSCs neural differentiation share common mechanisms with differentiation of primary neurons during development or differentiation of neural stem cells in vitro remains to be determined.
Among the Th differentiation programmes (arrows in Figure 5a), Th17 differentiation programme showed the highest positive correlation with Axis 1, and the iTreg differentiation programme strongly and negatively correlated with Axis 1.
The results showed that these hSKPs had multiple differentiation potentials.
The osteogenic- and adipogenic-induced differentiation experiments confirmed that the obtained ADSCs possessed multiple differentiation abilities.
The Th1 and Th2 differentiation programmes (explanatory variables) were correlated with their corresponding RNA-seq samples.
CCA triplot shows the correlations between genes, cell samples, and differentiation programmes.
In addition, we examined whether CCA can reveal hidden associations between genes, samples, and differentiation programmes.
