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Furthermore, when simultaneously express multiple different proteins, mRNA vectors allow for greater flexibility and control over stoichiometric ratios, dose titrations, and complete silencing of expressions.
This abstraction from specific residues is an enormous advantage for addressing questions involving multiple different proteins but might be disadvantageous for scoring in a traditional virtual screening scenario with only one protein.
These findings indicate that disordered IRs can provide the ability to interact with multiple different proteins.
Indeed, there are several scaffold proteins (e.g. Ste4, Ste5, Bem1) which bind multiple different proteins that could form a network of interactions, and in addition, there are also large structures such as the polarisome [9], [21] composed of many subunits that could give rise to highly cooperative behavior.
On the other hand, the complex RING E3s contain multiple different proteins.
Potentially, multiple different proteins could be produced from one mRNA and this has been experimentally confirmed for some transcripts (20, 32).
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We demonstrate using nanohole arrays of mixed designs and a microwriting process based on dip-pen nanolithography to monitor multiple, different protein binding events simultaneously in real-time based on the intensity of Extraordinary Optical Transmission of nanohole arrays.
Many neurodegenerative diseases, including PD and LBD, are marked by multiple different protein aggregates.
These maps can then be mined to identify common patterns of residues in contact that occur in multiple different protein structures [ 5].
Because spindle length is determined by the interplay of numerous molecular players, including multiple different motor proteins [ 62], it should also vary as a function of cytoplasmic protein composition, as illustrated by recent experiments comparing spindle lengths in different Xenopus species.
Using mass spectrometry analysis, we have detected multiple citrullinated peptides with C-terminal citrullines (as well as non-C terminal citrullines) in tryspin digests of multiple different citrullinated proteins in several experiments.
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