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After removal of soluble proteins using sodium dodecyl sulfate, confocal microscopy was used to visualize the 3D distribution of independent ECM networks in multiple developing tissues, including the forelimb, eye, and spinal cord.
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HOX gene expression in reproductive tissues was similar to those in the developing tissues, displaying ongoing proliferation, differentiation, and degeneration of multiple cell types.
For example, TC148, which encodes a barley cytosolic HSP90 homologue, was represented by multiple ESTs in all developing tissue libraries.
developed tissue preparation and staining protocols.
Both cytokines perform multiple functions in developing and adult tissues, including the brain, and also in a variety of tissues following injury.
The chicken-specific techniques will be of highest interest to investigators using avian embryos, development and packaging of lentiviral vectors that reliably express multiple proteins in infected cells should be of interest to all investigators whose experiments demand manipulation and expression of multiple proteins in developing cells and tissues.
Lmna-/ mice appear normal at birth but become runted as early as 2 weeks of age and develop multiple tissue defects that mimic some aspects of human laminopathies.
This is demonstrated in transgenic mice, which develop multiple tissue lesions including hepatic fibrosis as a consequence of elevated levels of TGF-β [ 7- 9].
For this, multiple di and tri-nucleotide repeat containing probes were used to screen for SSR containing genes from a large scale cDNA library developed from multiple source tissues of an Andean genepool genotype (G19833 from race Peru).
Nme protein accumulation is coincident with the functional differentiation of multiple epithelial tissues in the developing mouse [ 18].
They could develop into tissues other than cartilage, most commonly fibrous scar tissue".
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