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Multiple crossings were performed for twelve years among S. officinarum and S. spontaneum (Population 1) and between commercial varieties SP80-180 and SP80-4966 (Population 2) to generate genotypes with extreme values of sugar content.
Additionally, the finding that fish with multiple crossings were more frequent in couples than in the random pool, suggests that this feature enhances the chance of a fish to be chosen as a partner by conspecifics.
The frequency of occurrence of the multiple crossings were 9.5% and 21.2% in the random and the couple samples, respectively (n = 42 side photographs for the random, and n = 52 for the couple samples); and 2 of the 12 test fish sides used for the behavioral experiment showed multiple crossings, in both cases only on one side (16.7%).
Furthermore, the results suggest that multiple-crossings are relevant for partner selection in this species.
A set of 175 multiple crosses were made for each of the inter-crossing rounds.
The crossings are open for humanitarian needs.
Subaqueous crossings are becoming more ambitious.
Pedestrian crossings are almost totally disregarded.
All trains at main border crossings are now inspected.
Texas railroad crossings are the second-deadliest in the country.
And South-to-North crossings are extremely unusual.
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