We also map many more reads than the previous study for other multiple copy sequences, for example mir-92b and mir-87 (Fig. 2A).
We have compiled and curated the multiple copy sequences discovered using the above sequencing approach and phrap assembly into a soybean repeat database, available from the authors' web site [ 26].
The combination of a great reduction in unique sequence signatures and in the ratio of single copy to multiple copy sequences following 2 DPA suggests that there may be a change in the small RNA regulatory environment in cotton ovules once the early patterns are established.
Several microsatellites, notably PV-ag004 and PV-atct001, produced multiple banding, patterns especially in the wild beans which was interesting because these arcelin and phytohemagglutinin-based markers are part of sequences in the APA gene family which has several highly similar multiple copy sequences at least for the Arc5 [ 22].
Comparison of 454 survey sequences to previously sequenced BAC clones can reveal regions of multiple-copy sequence and allow approximate quantitation of copy number.
RIP targets multiple-copy sequences during meiosis and introduces cytosine (C) to thymine (T) mutations [ 51].
Of the 16 SSR primer pairs, seven, H1G20, H1C092, TA3, H1A19, H1H22, H1C9-2 and H2J2, were clearly derived from multiple-copy sequences, as indicated by Southern analysis and the library screening result that they hybridized to 47 - 672 positive clones, respectively.
Phylogenetic and comparative analyses with the multiple PwRn1 copy sequences will be informative to elucidate the evolutionary status/mode of the element among the Paragonimus populations with different polyploidy.
Multiple copies of sequences analogous to CRASP-1 genes have been detected in Borrelia plasmids.
All PCR products were cloned using the TOPO TA vector (Invitrogen) and multiple copies were sequenced on both strands.
