Sentence examples for multiple copy plasmid from inspiring English sources
A specific oligonucleotide probe AtzB1 was designed based on the sequence data of the atzB gene involved in the hydrolytic deamination of s-triazines; this gene, located in a multiple copy plasmid was detected by the optimized FISH protocol.
The resulting assemblies were used to confirm the orientation, bp deletion, and plasmid insertion copy number where applicable (not successful for multiple copy plasmid insertions) by alignment to the reference sequence Fusarium graminearum PH-1 genome and the plasmid sequence within Geneious version 7.1.6 created by Biomatters (available from http://www.geneious.com/) using LASTZ [ 39].
Coverage of the three DAF strains across the plasmid sequence was found to be variable with either some segments absent and/or other segments with increased read density indicating multiple copies of plasmid sequence at the insertion site.
P. aeruginosa is well known for harbouring multiple copies of plasmids, some of which are conjugative with resistance genes that are responsible for multiple drug resistance.
The activity of the introduced promoter may be higher than its actual level because of multiple copies of the plasmid.
Overexpression was achieved by the presence of multiple copies of the plasmid in transformed cells, as a result of selection for neomycin resistance by G418 in Dictyostelium [32].
Multiple copies of the plasmid may have been integrated.
Occasional preexisting cells have multiple copies of a plasmid that already carries an internal tandem duplication of lac.
This result is possibly explained by the presence of multiple copies of the plasmid present in the cell during the experimental trials.
The high XI activity was explained by integration into the genome in multiple copies of the plasmid carrying the XylA gene [ 51].
In future experiments it will be necessary to demonstrate that DECKO functions efficiently when expressed from a single genomic integration, as opposed to multiple copies of transfected plasmid.
