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IS6110 was chosen because of multiple copy numbers of IS6110 (6 to 24) in the MTB genome, making it an attractive target for PCR amplification [5].
Other studies described its inherent difficulties, e.g. low PCR success [9], [11], problem of secondary structure formation, resulting in poor quality sequence data [37], [38] and multiple copy numbers [48], etc.
They are maternally inherited as haploid genomes with multiple copy numbers in a cell.
In Halobacterium these elements have expanded to multiple families and subfamilies with multiple copy numbers [ 13].
If natural selection is partially blinded by the buffering effect of multiple copy numbers, we expect a high amount of nonsynonymous polymorphism to exist in mitochondrial populations.
This is in contrast with the traditional production of Tg mice which is based on random insertion of multiple copy numbers of transgenes [ 2].
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Figure 4 demonstrates the application of SiDCoN to interpreting the level of DNA copy number change in the presence of stroma (4b and 4c), and estimating the mixture levels of multiple copy number changes (4a and 4b).
However, after 5 days cultures only one copy number of SXT was detected in the E. coli transconjugant suggesting a decrease from a multiple copy number arranged in tandem to only one after this time [18].
The intensity of this band was higher than the observed in the wild-type cells, probably due to the low but still multiple copy number of the pBBR1/katE vector in Xac cells.
We identified multiple copy number alterations within this cytoband and defined distinct minimal regions of alteration (Additional file 11).
Genomic context analysis revealed that CTLP rearrangements frequently occurred in genomes that additionally harbored multiple copy number aberrations (CNAs).
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multiple copy aberrations
multiple research numbers
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