Sentence examples for multiple copy integration from inspiring English sources

A series of integration expression vectors, pxKC3T5b and pxKT5b, were constructed for chemically inducible chromosomal evolution (multiple copy integration) and replicon-free and markerless chromosomal integration (single copy integration), respectively.

This identified one single copy integration for all the lines but one multiple copy integration for line 8/S1 (data not shown).

These A. niger strains lack the catalytic subunit (strain Δ pkaC) or have a multiple copy integration of the catalytic and the regulatory subunit (mc pkaCR).

The gene AraB from E. coli was integrated in a single copy [ 26, 27], while B. subtilis AraA and E. coli AraD where targeted to the rDNA region of S. cerevisiae to allow multiple copy integration [ 27].

Little is known about silencing of SIN lentiviral vectors in ES cells but it has been shown that transgenes are efficiently expressed at multiple copy integrations but single copy integrations results in inconsistent expression [12], [13], [23], [28].

Further inverse metabolic engineering based on functional complementation suggested that efficient xylose assimilation is attributed, in part, to the elevated expression level of xylose isomerase, which was accomplished through the multiple-copy integration of XYLA in the chromosome of the evolved strain.

A high density of A. tumefaciens increased the transformation frequency; however, undesirable integrations of the T-DNA such as multiple-copy integrations at a single locus, with or without the vector backbone, or integration at multiple loci also increased in A. aculeatus, as previously reported in Blastomyces dermatitidis (Sullivan et al. 2002) and in Suillus bovinus (Hanif et al. 2002).

The P. pastoris transformant that could resist higher concentrations of geneticin G418 might contain multiple copies of integration of a heterologous gene into the P. pastoris genome, which could potentially lead to a higher expression level of the heterologous protein as elucidated in the manual of the Multi-Copy Pichia Expression Kit (Invitrogen, Carlsbad, CA, USA).

The results indicate that integration of the gene cassette into multiple copy loci should be given consideration when designing amylolytic yeast strains.

Successful homologous integration was tested by colony PCR and single copy integration was verified via Southern blot analysis.

U: unlinked In the present study, we developed an alternative method to promote low copy/single copy integration using UV/TMP.