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The integration of multiple copies was confirmed by genomic Southern blot analysis.
The nuclear region distributed with nuclear ribosomal RNA genes (rDNA), which are organized in tandem arrays of multiple copies, was probed by fluorescence in situ hybridization (FISH) (Fig. 4m x).
In a study of human segmental duplications, the 'ancestral copy' (positional ortholog) for a segment with multiple copies was determined using BRH nucleotide-level alignments [ 84].
To filter for possible paralogous sequences in all datasets, the subset of all possible paralogs/orthologs that have the smallest reciprocal distance and that included all species having multiple copies was chosen.
However, the number of assembled contigs was sufficient to indicate the number of gene copies, and when evidence for multiple copies was obtained, individual genes were accurately assembled by a manual process.
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Multiple copies were then routed to multiple record centers.
Multiple copies are held at one or more data centers.
With the exception of the TATA box, only those with multiple copies were counted.
These multiple copies are often colocalized in interphase, suggesting either an ectopic association or a highly reproducible interphase chromatin configuration.
When reconstructing the watermark, we employ a majority voting scheme to verify each retrieved bit in cases where multiple copies are received.
cis-elements within motif sequences were counted for all putative regulatory sequences in the training set, and those with multiple copies were summarized (Table 2).
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