Exact(4)
Multiple controls were matched to each case for gender, age, diagnosis, hospital, operation date, follow-up, stem type, and cup design.
(Of note when multiple controls were used in the same reaction run the KCt/control was obtained by averaging all the controls' "Corrected Ct" – KCt/control's).
Multiple controls were printed on each protein array chip to evaluate detection conditions and background signal (Additional file 1, Figure S1).
Multiple controls were matched to cases by age, census tract, and urban median household income quintile/rural residence at January 1 , 2008
Similar(56)
Our results provide evidence that the selection of multiple controls is crucial to prevent the widespread risk of Type II error, highlighting the need of more accurate experimental designs when dealing with meiofauna.
Multiple controls are needed for that.
Multiple controls are required to separate these interacting factors, and studies that do not include all of the appropriate controls cannot be easily interpreted.
We conclude that the identification of context-specific reference genes, combined with existing methods for normalization against multiple controls, is expected to significantly improve the quality and sensitivity of expression quantification experiments, facilitating the correct interpretation of RT-qPCR data.
Multiple negative controls were included in each extraction.
If multiple potential controls were identified, two were selected at random.
Multiple negative controls were included and DNA from Cp. abortus and C. suis (kindly provided by K. Sachse, Friedrich-Loeffler-Institute, Jena, Germany) were used as positive controls.
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