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Additional challenges include the following: the high cost per assay to the user, lengthy sample preparation steps, and multiple control tests that need to be performed separately.
However, this method can grow increasingly complex with the number of proteins that need to be screened simultaneously: spectral emission overlap of fluorophores and the subsequent need for compensation, lengthy sample preparation, and multiple control tests that need to be performed separately must all be considered.
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Multiple control test sections were also built to evaluate the effect that subgrade strength, base course thickness, and/or presence of the geosynthetic had on performance.
Statistical analyses were performed using unpaired t-tests (for comparing two groups) or ANOVA tests, followed by either Dunnett (for multiple control vs test comparisons) or Student-Newman-Keuls (for multiple comparisons) post-tests.
Statistical analysis was performed using unpaired t tests (for comparing two groups) or analysis of variance tests followed by Dunnett (for multiple control versus test comparisons).
Western blots that were quantitatively compared with multiple control and test samples were all run on the same resolving gel.
It is important to point out that these are reversible inactivations, not permanent lesions, allowing us to collect multiple control and test conditions within each animal.
These observations were consistent in samples taken from multiple patients' and controls tested in parallel against multiple allogeneic donor cell populations (Fig 4A D).
We also provided flexible missing value options and integrated efficient multiple testing control methods for the new eLSA technique.
Edge scores cannot be computed when an overly strict multiple testing control results in no causal anchors.
On the other hand, an overly lax multiple testing control may result in spurious causal anchors which may lead to erroneous edge scores.
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