Sentence examples for multiple control genes from inspiring English sources

In another approach, the geometric average of multiple control genes was found to be an accurate normalization factor for RT-PCR measurements [29].

Normalization was carried out using multiple control genes as described by Burton et al. [ 91].

Choice of nCounter control genes changed correlation results slightly, with multiple control genes generally performing better than single control genes, as previously suggested for other technologies [ 37].

Cautionary notes: As for quantitative PCR experiments in general, it is important to normalize gene expression to multiple control genes (e.g.,, act-1, cyn-1, ama-1, nhr-23, pmp-3, and cdc-42).

For each patient, tumour vs. normal fold change was determined based on the method of Antonov et al whereby the Ct of the test gene is normalised by the geometric mean of multiple control genes [ 9].

Whereas for qRT-PCR establishing a set of multiple control genes per tissue and condition becomes more and more good practice, this is not (yet) at all the case for western blot studies.

Where indicated, microarray expression values were similarly normalized to single or multiple control gene expression values.

The relative expression of BRCA1 was determined using the free access qBase software (Hellemans et al, 2007), which is based on a modification of the classic delta delta Ct method that allows for PCR efficiency correction and multiple control gene normalisation.

However, the accuracy of this technique heavily relies on the use of multiple valid control genes for normalization.

The method described in GeNorm [42] was used to derive normalization factors from the multiple internal control genes and relative quantification was performed using the 2−ΔΔCt method.

Multiple reference control genes were tested against all samples to identify control genes whose expression was not affected by the NH4+ treatments.