Sentence examples for multiple contigs were from inspiring English sources
Individual clusters which mapped to multiple loci and/or arose from cluster assemblies producing multiple contigs were omitted to reduce the mapping of clusters potentially containing EST reads of duplicate genes and closely related members of gene families.
Multiple contigs were often assembled, that frequently contained BAC clones from a single haplotype.
Step4 Reclustering and reassembling were performed under more stringent conditions if multiple contigs were generated.
Sequences were further filtered so that the local database contained only 1 unique contig for each gene segment, and multiple contigs were generated for each sample.
When mega-amplicons representing the 24 patient samples were tested in the NGS, a total of 32.8 million reads were obtained, and multiple contigs were generated for each sample (Technical Appendix Table 3).
We excluded clusters containing singlet sequences for obvious reasons; we considered only one contig per cluster when multiple contigs were present to avoid redundancy; a mismatch was regarded as SNP candidate if it appeared at least twice in the alignment.
99.8% of these events contained grouped exons that were all on the same contig (557/558); the single event that grouped exons from multiple contigs was removed from the analysis.
This suggests that multiple contigs are formed for individual genes because of assembly of incomplete reads; these duplicate contigs represent redundancy in the contig assembly.
The annotation and analysis of signatures matching multiple contigs is a more difficult task, but validation of these data could be performed by using microarrays with specifically designed probes to determine the relative expression of all matched genes, or by repeating the MPSS experiment using a different "anchoring enzyme" such as NlaIII (CATG) instead of DpnII (GATC).
Multiple marker contigs were distributed across all 29 LGs.
Therefore, the genes with hits of multiple genomic contigs were used as a starting point for further analysis and visual inspections.
