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Exact(6)
Contig clustering allowed for multiple contigs to be assigned to a single phage genome.
The mappings of multiple contigs to the guide protein can then be viewed as a graph (Fig. 3).
Next, we order and orient these contigs by mapping them to proteins, allowing multiple contigs to map to the same protein.
This ensured that the BLAST output only reported the most significantly similar match between each contig and the query that matched it, but allowed multiple contigs to be matched by the same known query.
Complete assembly of a typical bacterial or archaeal genome of several megabases is usually impractical, even when extremely deep sequencing is performed, and assignment of multiple contigs to a single genome is a separate, non-trivial problem.
A network matching algorithm is proposed for matching the de Bruijn graph of contigs against reference genes, to derive 'gene paths' in the graph (sequences of contigs containing gene fragments) that have the highest similarities to known genes, allowing gene fragments contained in multiple contigs to be connected to form more complete (or intact) genes.
Similar(54)
A total of 8.1 genome equivalents of Illumina reads were mapped onto the WGSAS, assigning reads that map to multiple contigs randomly to one of the possible contigs.
The discordance between the number of contigs and the number of unique sequences they aligned with is due to multiple contigs mapping to the same gene.
Multiple contigs mapping to different parts of the same reference were merged to give a single gene model, with gaps filled with runs of 'n'.
If multiple contigs belonged to a cluster, only the longest was retained in the reference transcriptome.
When multiple contigs mapped to different sections of the same gene/Unigene, the sequences were condensed using a Perl script into a single model, with gaps filled by strings of 'n'.
Related(20)
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