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Second, EST assembly sometimes resulted in multiple contigs that contained an identical CDS.
Multiple contigs that retrieved the same Arabidopsis AGI-code were treated as partial transcripts of the same gene.
The multiple contigs that were assembled into unigene clusters may represent transcription variants, allelic variants, closely related paralogous sequences, misassembled transcripts, or transcripts that were fragmented due to low coverage [ 46].
Coupled with our failure to find a PXR-like gene in the preliminary release of the sea lamprey genome (despite finding multiple contigs that contained fragments of a putative FXR gene in addition to the already characterized VDR gene), the available data suggest that PXR gene(s) may not exist in the sea lamprey.
For example, it remains unclear both why reads from some genes assembled into multiple contigs that clustered based on regions of nucleotide identity (up to 230 contigs in a cluster) and why a few accession numbers have so many representative transcripts aligning to them.
Highly divergent families or families with a large number of divergent subfamilies that each have low copy numbers would not assemble well in our analysis or produce multiple contigs that all fall below the 0.01% cutoff.
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The Geneious assembly of multiple Velvet contigs that were constructed under different parameters would identify any Velvet contigs that were improperly assembled.
As observed in Arabidopsis and other species, the multiple contig copies that comprise many of the loci may represent splice variants, not fully sequenced transcripts or different alleles.
For large sequence contigs that matched multiple WGP contigs, the WGP contigs were ordered according to the tag positions on the sequence.
Using the L. goodei 454 contigs as a reference anchored the analysis of shorter Illumina sequences and allowed identification of contigs that contained multiple SNPs.
To test these predictions we randomly chose ctg3184 (which contains 166 clones) from among those contigs that had multiple probe hybridizations.
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