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Data from baculovirus infection expression trials for multiple constructs of two target protein systems relevant to the study of human diseases are presented.
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Overexpression of multiple constructs (DsRed and GCaMP2) had no deleterious effect on rates of postcrossing axon outgrowth, which grew at 114% of the rate of controls expressing only one construct (a nonsignificant increase).
These have involved both multiple integrations of one construct into different locations [ 75] or integration of multiple constructs into one site on the X chromosome [ 13].
The methods for parallel GATEWAY cloning and expression of multiple constructs in cell culture will also be useful for applications such as the generation of receptor protein microarrays.
In the Rasch analysis, the PDI and its subscales appeared to measure multiple constructs, making the validity of deriving a single overall score questionable.
Distributed cones and fields are maars, cinder cones, spatter cones, and shields or fields of multiple constructs.
This eliminates the need for additional subcloning and isolation steps and also the need for the sequencing of multiple constructs.
This single-item approach promotes efficient and effective measurement of multiple constructs when sensitivity to change is not a primary methodological priority.
It has also been argued that the BI is a multidimensional scale (i.e. measures multiple constructs) and consequently summation of BI item scores to obtain a total score does not yield an interpretable index [ 8].
In conclusion, this study has led to better understandings about participants' multiple, constructed realities of leadership and followership in different healthcare contexts.
Simultaneous reporting for multiple DSBR pathways can be achieved by integrating multiple constructs into a single clone (20, 23, 24) or by integrating a single reporter construct that detects multiple types of repair (25, 26).
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