Sentence examples for multiple consensus sites from inspiring English sources

Site-specific mutagenesis of eight Asn-linked glycan consensus sites (Asn to Gln mutations) suggested that PLD4 was exogenously expressed in HEK293 cells and contained large N-glycosylated moieties at multiple consensus sites (Fig. S5B).

This region contains multiple consensus sites of serine phosphorylation for casein kinase 2 (CK2), which phosphorylates MAP1B and enhances its binding to microtubules.

The human ER beta promoter contains multiple consensus sites for several transcription factors, including an AP-1 box, OCT-1, GATA, and ARE [ 39].

δEF1 has multiple consensus sites for phosphorylation by Erk kinases (Costantino et al, 2002), and the bands corresponding to δEF1 by SDS PAGE migrated faster after in vitro dephosphorylation by alkaline phosphatase (see top right panel in Supplementary Figure S3E).

The 6xOSE2 promoter sequence, which contains multiple consensus sites for RUNX2 binding (41), was synthesized (GeneArt, Invitrogen) and subcloned into the HindIII restriction site of the pGL3-Basic expression vector (e1751; Promega) directly upstream of the firefly luciferase coding sequence.

Because N-glycosylation efficiencies of NXS consensus sites are more sensitive to the middle X residue, we settled on E2 and E4 constructs that had a single N-terminal NXS consensus site, which (i) simplifies the kinetic measurements and (ii) eliminates any cooperativity caused by the presence of multiple consensus sites.

Exon 4 gives rise to the isoform RCAN1.4, and is under the control of a calcineurin responsive promoter, comprising multiple consensus binding sites for the transcription factor nuclear factor of activated T-cells (NFAT) [9] and GATA-2/3 sites [10].

As shown in Fig. 1, both Gab1 and Gab2 as well as DOS contain multiple consensus binding sites (Y-X-X-P) for the SH2 domain of Crk proteins [ 127, 129].

Characterization of the PfLAMMER describes the enzyme as comprised of 2 domains, where the N-terminal domain is unique and containing multiple consensus phosphorylation sites, a number of RS/SR dipeptides, a large portion of charged residues, two putative nuclear localization signals, and 14 copies of a DKYD repeat and the C-terminal domain is typical of the LAMMER family [ 42].

Among the 15 consensus GSK3β sites on BMAL1, we focused our attention on two loci which contained multiple GSK3β consensus sites in tandem.

These findings suggest that phosphorylation of TBC1D1 at multiple AMPK consensus sites is necessary for normal contraction-stimulated glucose uptake in skeletal muscle.