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By comparing multiple confocal z-sections, we found that vacuoles could be associated with either the ventral or dorsal surface membrane of the growth cone.
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At least 3 non-overlapping optical sections were sampled from confocal Z-sections taken from 3 individual specimens.
We imaged embryos that were "flattened" to maximize the number of nuclei that could be captured by Confocal z-sections for each embryo.
Images are maximum projections of confocal Z-sections.
(A ) Projections of confocal Z-sections through entire pachytene nuclei.
Images in (A ) and (C ) are maximum projections of confocal Z-sections.
Confocal z-sectioning shows that epithelial and smooth muscle cells tend to separate into two distinct layers on a single nanofiber system mimicking the in vivo anatomy.
CRIB also affected the distribution of vesicles in cells as examined by confocal Z-sectioning of cells (Fig. S5).
Confocal Z-sectioning of the salivary glands provided views suggesting that the organisms were present within the salivary glands and not located externally (Figure 3).
Each picture is a stack of merged confocal Z sections.
Each picture is a stack of merged confocal Z sections in all channels.
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