Furthermore, we demonstrated that this method outperforms our recently developed somatic TALEN technique and enables the one-step generation of multiple conditional knockouts.
To circumvent these limitations and study abnormalities in postnatal mice, multiple conditional-knockout models of TSC have been established by inactivating the Tsc1 or Tsc2 genes in specific cell types, including in neurons and astrocytes (Uhlmann et al., 2002b; Way et al., 2009; Wong et al., 2003).
Future studies using multiple and/or conditional knockout mouse models will be essential to finally demonstrate their direct role in tumour suppression.
In mice alone, multiple targeted transgenics, chimeras, a conditional knockout, and a regulable cell-type-specific disease-rescue model have been engineered.
Similar conditions developed in hepatocytes of Atg7 conditional knockout mice.
The mice were intercrossed through multiple generations to create colonies of homozygous Brca1loxP/loxP/p53 loxP/loxP, RbloxP/loxP/p53loxP/loxP, and RbloxP/loxP/Brca1loxP/loxP double conditional knockout mice.
In this chapter, the types of genetically engineered mice, like transgenic, knockout, conditional knockout and knock-in animals, are presented.
Our approach reduces the time and resources required for conditional knockout mESC generation dramatically.
We determined that these conditional knockout mice, designated Gjb2-CKO, have a severe hearing loss.
We also identified changes in the epithelial cell populations present in E-cadherin conditional knockout animals.
