This is addressed by employing a diffuse interface model with multiple concentrations.
In another study, Griner et al. conducted a large scale screen of multiple concentrations of 500 compounds with ibrutinib in activated B-cell-like diffuse large lymphoma cells [74].
For confirmatory assays, active and inactive statuses were defined based on EC50/IC50 values, derived from dose response curves following testing with multiple concentrations.
Naked DNA was digested with multiple concentrations of DNase I to rule out sequence-based bias of DNase I digestion [ 4].
Then, the data were analyzed by nonlinear regression fit of association kinetics with multiple concentrations in Graph Pad Prism 6. A431 cells (ATCC) were cultured in DMEM (Thermo Fisher) supplemented with 10% fetal bovine serum (FisherBrand).
Vehicle controls received sterile water at the same volume as for acrolein treatment (e.g., for the 25-μM acrolein dose, controls received 25 μL of water into 10 mL of complete medium); for comparisons with multiple concentrations of acrolein, the vehicle control received the volume of water used for the highest acrolein dose.
A precise LC50 value (2.95 × 102 conidia ml−1) was calculated for HRI 1.72 using a second multiple concentration assay with smaller concentrations of conidia.
By contrast, the TCAD regimen was synergistic against all three viruses over multiple concentrations of all three drugs, with the synergy occurring at 0.1 µg/mL and above for amantadine, 0.32 µg/mL and above for ribavirin, and 0.0032 µg/mL and above for oseltamivir carboxylate (Table 1).
Multiple concentrations of purified VBC complex were diluted in kinetics buffer and allowed to associate with immobilized peptide over 120 s.
We study the existence of sign-changing solutions with multiple concentration to the following boundary value problem−Δu="ε2|x|2α eu−e−u) in Ω,u="0 on ∂Ω, where α>0, Ω is a smooth bounded domain in R2 containing the origin, ε>0 is a small parameter.
