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To screen multiple concentrations of each compound in a rapid and sensitive manner, we took advantage of automated fluorescence microscopy18,21,37,38 (Fig. 1a).
Multiple concentrations of purified VBC complex were diluted in kinetics buffer and allowed to associate with immobilized peptide over 120 s.
Multiple concentrations of dusts and a stoichiometric methane mixture were tested.
Cultures were exposed for 24 h to multiple concentrations of flusilazole (0.54 54 μM) and RNA was isolated.
In another study, Griner et al. conducted a large scale screen of multiple concentrations of 500 compounds with ibrutinib in activated B-cell-like diffuse large lymphoma cells [74].
Multiple concentrations of inhibitors were initially screened as part of the intracellular calcium studies [17], [37].
Similar(23)
(A ) Overlay of SEC-MALS chromatograms of SAS-5Imp in multiple concentrations showing formation of a stable dimer.
Analysis of multiple concentrations gave a molecular weight of 17,727±± 219 Da, close to the expected molecular weight for the monomer of 19,640 Da.
For a given protein, data for each residue can be measured at multiple field strengths and multiple concentrations, creating an abundance of data.
In contrast, a single array DRC experiment consists of multiple concentrations and multiple external RNA controls per concentration, all tested on a single array.
The 10-s PM2.5 averages during the smoking periods showed great variability, with multiple high concentrations of short duration (microplumes) both indoors and outdoors.
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