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Since these assays are endpoint assays, we performed the analysis at the 47-h time point since performing these assays over time would require another full screen of 308 compounds at multiple concentrations for each time point.
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All camptothecin analogues studied (at multiple concentration for each drug) had a peak at chromosome 20 centered around 50 cM (Figure 2, Dataset S2).
For those studies reporting data for multiple concentrations of a particular Cry protein, we included data for only the highest reported dosage.
In this study we have analysed the transcriptomes of two distinct human cell lines, MCF-7 derived from a breast carcinoma and HepG2 originating from a hepatocellular carcinoma, after exposure to multiple concentrations of BaP and for different lengths of time to identify the relationship between these variables and gene expression modulation.
Cultures were grown with 1 ng/mL LIF for 12 days in vitro and then exposed to multiple concentrations of the test chemicals for 4, 24, 48, and 96 hours.
Vehicle controls received sterile water at the same volume as for acrolein treatment (e.g., for the 25-μM acrolein dose, controls received 25 μL of water into 10 mL of complete medium); for comparisons with multiple concentrations of acrolein, the vehicle control received the volume of water used for the highest acrolein dose.
Here we screened multiple concentrations of four tubulin-binding drugs for their ability to rescue levels of acetylated α-tubulin in patient-derived ONS cells.
Cultures were exposed for 24 h to multiple concentrations of flusilazole (0.54 54 μM) and RNA was isolated.
Stopped-flow traces for the binding of multiple concentrations of each catecholamine to PheH were fit globally using KinTek Explorer.
Multiple concentrations of bentonite and MgCl2 were investigated in pH 4.0 buffer to determine the optimal concentration for removal of TMV CP.
Multiple concentrations (2.5; 5; 10 μM for EA and 2.7; 13.7; 27.5; 55 μM for RA) were prepared in dimethylsulfoxide (DMSO, Sigma-Aldrich, St . Louis MO, USA) and diluted in DMEM so that the final concentration of DMSO in the medium was 0.1% for EA and 0.5% for RA.
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