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Following the initial automated screen, the images of the affected cells were inspected manually and a secondary screen was performed, in which hit compounds were tested at multiple concentrations, and directly compared to the well-established proteasome inhibitor MG132.
The following eight class-comparison analyses were conducted to infer genes differentially expressed in response to TNT or RDX: (1) two 2-class comparisons: pooled controls vs. pooled TNT or RDX treatments; and (2) six multiple-class comparisons: 4-day TNT or RDX multiple concentrations, 4-day repeat TNT or RDX multiple concentrations, and 14-day TNT or RDX multiple concentrations.
In contrast, a single array DRC experiment consists of multiple concentrations and multiple external RNA controls per concentration, all tested on a single array.
By applying a large sample size, multiple concentrations and two time points, we found little evidence for CSC-specific profiles for the three brands analyzed.
(A ) Overlay of SEC-MALS chromatograms of MsyB-tagged SAS-5FLEX in multiple concentrations and (B ) plot of average molecular weight from SEC-MALS analysis as a function of on-column protein concentration.
Calibration was performed on a daily basis with authentic standards at multiple concentrations, and the experimental standards were diluted so that the areas of all peaks fell within the calibration range.
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These low-dimensional models are described by multiple concentration and temperature modes (variables), each of which is representative of a physical scale of the system.
Experimental benchmarking of the develop framework was undertaken using the only published multiple concentration and incident photon spectra experimental NP radiosensitisation study; the Ph.D. thesis of Rahman, RMIT University (Australia) (Rahman 2010).
In this study we have analysed the transcriptomes of two distinct human cell lines, MCF-7 derived from a breast carcinoma and HepG2 originating from a hepatocellular carcinoma, after exposure to multiple concentrations of BaP and for different lengths of time to identify the relationship between these variables and gene expression modulation.
Multiple concentrations of dusts and a stoichiometric methane mixture were tested.
Each TLR ligand was tested at multiple concentrations (not shown) and results are shown using an optimal concentration for each agonist.
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