Post-hoc multiple comparisons utilizing the Scheffe test was performed to determine where the differences existed.
When a global difference over time was observed, Bonferroni post-hoc analysis with correction for the effect of multiple comparisons was utilized as 0.05/n, where n is the number of comparisons.
Independent samples T test between two groups and single factor analysis of variance (ANOVA) for multiple comparisons were utilized when the data in accordance with normal distribution and homogeneity of variance.
Statistical comparisons utilized within subjects designs; simple comparisons utilized paired (dependent) T tests.
Comparisons utilized continuous laboratory units and statistically standardized z-scores.
For the real-time RT-PCR assays, a Kruskal-Wallis test and Dunn's multiple comparisons tests were utilized with the level of significance set at P<0.05.
The Nemenyi multiple comparison test was utilized to determine statistical differences among the cups, and it found that samples from the Green Decorative mug were statistically significantly higher than samples from all other cups (p = 0.0001 0.0125).
For water maze analysis, a repeated-measures ANOVA and post hoc t-tests (including Tukey's multiple comparison corrections) were utilized.
The resulting P values have to be corrected for multiple comparisons (e.g., by utilizing the false discovery rate (FDR) [ 78]), followed by correction for multiple comparisons at cluster level [ 79].
