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Exact(6)

uPAR-positive cells isolated from three SCLC lines were able to proliferate and form multiple colonies in methylcellulose media, while uPAR-negative cells displayed little or no clonogenic potential.

Stable Oct4-GFP activity emerged in multiple colonies in serum-free medium with LIF within 7 days.

Analysis of bird-borne tracking data indicated that 25percentnt of their North American population from multiple colonies in eastern Canada migrated to the pollution zone.

Significantly, after just 14 days in co-culture the primary tumor cells derived directly from clinical specimens of ductal carcinoma in situ (Fig. 1c) or invasive ductal carcinoma (Fig. 1d) also produced multiple colonies in close proximity to fibroblasts.

Indeed, ESRRB expression rendered reset cells resistant to TFCP2L1 shRNA such that they formed multiple colonies in t2iL+Gö that had refractile domed morphology and could be expanded after passaging.

Genomic DNA was obtained by boiling a sample obtained by streaking from multiple colonies in 0.05 mol/L NaOH for 2 min. The crude cell lysates were centrifuged for 2 min at 2,000 × g, and 2 μL of the lysate was used in PCRs.

Similar(54)

The mock transfected cells all died, but multiple colonies survived in the transfected cultures.

Gamete bundles from multiple colonies (and in the case of A. palmata, from multiple reefs) were combined within an hour to obtain cross-fertilization among different genets (these hermaphroditic species do not self-fertilize).

For the mt lariat RT-PCR products, we identified 15 colonies with the insertion, of which the -22A, -18A and -15A were identified from multiple colonies for in branching reaction of mt BRAF RNA splicing (Fig.  4c, d) and the -20A and -26A were found each only in a single colony.

(Cultures grown from a single colony of MJ11 were as heterogeneous in light output as cultures grown from multiple colonies, as described in Text S1).

It is normally only recommended if multiple colonies are found in a single structure.

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