The 64 codons encode 20 different amino acids and three termination signals.
Both codons encode leucine.
However, these 35 codons encode all amino acids with synonymous codons.
There are six sets of codons encoding arginine, which poses considerable challenge to ArgRS insofar as identifying cognate tRNA via matching the anti-codons.
Insertion of two codons encoding a stop codon in the + 1 frame before four-base codon suppressed the expression of non-labeled proteins that may have been produced by spontaneous + 1 frameshift upstream of the four-base codon.
The phenomenon of unequal usage of synonymous codons encoding an amino acid in which some codons are more preferred to others is the codon usage bias (CUB) and it is species specific.
The codons encoding one amino acid may differ in any of their three positions.
There are 32 possible codons resulting from the NNK motif: unique codons encoding 12 amino acids, 2 codons encoding 5 amino acids each, 3 codons encoding 3 amino acids each, and 1 stop codon (amber).
At most half the codons encoding for a given amino acid are major codons.
We found a much greater density of small footprints at codons encoding smaller, polar amino acids than at codons encoding large, aromatic amino acids.
