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Sometimes, there might be multiple coding regions in the same DNA sequence containing that EST sequence.
The major RPL24B paralog, RPL24A, has also been implicated in the translation reinitiation on mRNAs with multiple coding regions [ 22].
Larger sequences where multiple coding regions are expected would need to be investigated with a sliding window.
However, incongruent results may be obtained from different typing methods based on either single or multiple coding regions of the genome [ 16- 19].
Given a nucleic acid sequence, ESTScan uses the Viterbi decoding algorithm to determine the most probable path through the model and to infer the most likely coding region or identify multiple coding regions.
In the 19,210 contigs that were input into ESTScan, 17,388 coding regions were identified across 16,982 contigs (a number of longer contigs were identified as containing multiple coding regions).
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For instance, 21 positions, including multiple coding region positions, have a higher frequency of PHP than position 16,093 – which past studies have repeatedly demonstrated has the highest rate of PHP in the CR [10,18,19,68,71,80].
CNV can affect gene expression levels, since it contains or disrupts multiple gene coding regions or regulatory elements, which could then lead to phenotypic variation [ 19].
The lengths of 23S rRNA and 16S rRNA sequences are approximately 3.0 kbp and 1.4 kbp, respectively, and the mean read length obtained using PacBio was 3.1 kbp, making it possible to correctly determine the absolute positions of multiple rRNA coding regions.
Unlike the clinically relevant mutation spectrum of genes currently analysed on FFPE tumour DNA, such as KRAS or EGFR, where the distribution and number of mutations is small, thousands of clinically relevant variations in BRCA1 and BRCA2 have been described, and these are distributed widely throughout multiple, large coding regions and intron-exon boundaries [ 17].
We observed that multiple rRNA coding regions were fragmented in the assemblies from the second-generation sequencers, whereas PacBio generated two exceptionally long contigs of 3,288,561 and 1,875,537 bps, each of which was from a single chromosome, with 73× coverage and mean read length 3,119 bp, allowing us to determine the absolute positions of all rRNA operons.
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numerous coding regions
various coding regions
many coding regions
several coding regions
multiple coding mini-
multiple coding genes
multiple brain regions
multiple ellipsoid regions
multiple coding units
multiple coding mutations
multiple coding variants
multiple target regions
multiple coding evens
multiple coding modes
multiple repeat regions
multiple coding frameworks
multiple coding categories
multiple coding exons
multiple coding sequences
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