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Since in our two hybrid screen two separate hTTP baits containing C-terminal hTTP sequences identified multiple clones representing two fragments of human CIN85 (aa 4 264 and aa 40 458), we predicted that the C-terminal region of hTTP was involved in its binding to the N-terminus of CIN85.
Multiple clones representing each of the 6 synthesized genes described in this manuscript were sequenced and the types or errors tabulated.
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Average fold differences were calculated when multiple clones represented a transcript.
Using individual clones prevents the assembly of chimeric FLcDNA sequences consisting of ESTs from multiple cDNA clones representing closely related genes.
It has been suggested that in multiple-clone infections, clones representing a minority of the total parasite population in a host might escape detection by PCR [32].
Positive controls included 132 β-actin clones and 111 clones representing 12 other ubiquitously-expressed genes.
Since multiple clones can map to the same locus, the 31,708 clones represent 15,552 distinct genes.
Because multiple twin spots are adjacent to one another, it is unclear if clones represent a single progenitor labeling event or more than one progenitor labeling events.
These six ES clones represent four different genes.
These clones represent paralogs of A. thaliana thionins.
Therefore, type II clones represent asymmetric neurogenic clones.
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