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These EVP cells give rise to TA and D cells that form mostly venous/arterial capillaries but also lymphatics through the combined contribution of multiple clones of endothelial cells.
The plasmids were extracted with plasmid DNA extraction kit Mini Plus™ (Viogene-Biotek Corp .. Multiple clones of a PCR product were sequenced.
With this new strategy, we engage the multiple clones of the entire immune system against cancer targets; thus greatly amplify the therapeutic efficacy.
In it, an attacker captures a node (most probably a sensor node in a wireless sensor network) and creates multiple clones of that physical node.
If we have computers we'll mainly be offered multiple clones of successful books - and food porn, design porn, 50 shades of soft porn.
Therefore, memory cells carrying the codes for polyclonal antibodies targeting multiple domains of the HBsAg produce multiple clones of antibodies, while all targeted towards the same cancer cells tagged by the variable fragments of AVEC.
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By D10, both venous capillaries and arterial vessels seemed composed of more than one color clearly suggesting the contribution of multiple clones to different sections of each vessel within the tumor.
This emphasizes the importance of using multiple clones for this type of studies.
Multiple clones (minimum of ten) from each PCR product were submitted for DNA sequencing at the Center for Genome Research & Biocomputing Core Laboratories Oregon State Universityy, Corvallis, OR).
An artificial stop codon was introduced at the 3' end of the PCR product and the fragment was cloned into the BamHI and SacII sites of one of the multiple cloning sites of the vector resulting in b3D-LplA1-int1.
Upstream of the multiple cloning site of pBBR1MCS-2 is a P lac promoter that typically allows for expression of cloned genes in E. coli ([Kovach et al. 1995]).
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