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Exact(19)
Multiple clones from each gene were sequenced.
Multiple clones (from at least two different PCR reactions) were selected and sequenced in both directions to verify sequence.
Given that the present results are all based on multiple clones from PCRs of extracts from at least two (usually three) teeth the likelihood of the ancient sequences presented here being due to artefacts seems very low.
Multiple clones from each PCR were sequenced as described above.
Most mutants were obtained as multiple clones from independent experiments.
Multiple clones from the same transfection were pooled and grown under puromycin selection.
Similar(41)
Finally, each base of K. setae NBRC 14216T genome was ensured to be sequenced from multiple clones and from both directions with Phrap quality score ≥70 or from one direction with Phrap quality score ≥40.
Multiple clones (29/72 from F2, 21/72 from F3) showed best-parent heterosis (that is, they showed higher ethanol tolerance than all parental strains).
Multiple clones, isolated from soft agar studies and low density plating, showed decreased doubling times.
We obtained this sequence by analyzing multiple clones (7) from four individual animals.
For this reason, we directly sequenced PCR amplified virus population cDNAs rather than sequencing multiple clones isolated from the population.
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