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To identify single nucleotide polymorphisms (SNPs), plasmid DNA for multiple clones for each product was double-digested with BglII to excise the cloned insert, and with HinfI, a frequent cutter, to generate a restriction fragment profile for each clone.
This emphasizes the importance of using multiple clones for this type of studies.
Multiple clones for each allele were sequenced to assure against PCR-introduced changes.
The SBE protocol was faster, cheaper and more accurate than sequencing multiple clones for SNP detection in historic specimens.
For rapid characterization of the overall synteny, minimal initial screening of the BAC library was carried out, and, in principle, we did not isolate multiple clones for each STS.
Multiple clones for each single cell population were sequenced to confirm disruption of NPC1 alleles.
Similar(46)
The clonal nature of these irises allowed for multiple clones of genetically identical plants to be used to generate the backcross populations.
This means that each patient or individual is represented by a single bacterial isolate (i.e. single clone type) as usually routine microbial diagnosis does not account for multiple clones of an identified bacterial species.
Herein, we present a way to by-pass the need for developing multiple clones of antibodies against HER-2, but rather we use HBsAg, which serves as a lightning rod for attracting all the clones of antibodies generated by immunization.
Thus, for example, multiple clones of JHE were found in the fat body library, consistent with the high level of expression of jhe in the fat body of the final instar stage larva [ 26, 27].
The MSLL clones were complete digests, so there should not be multiple clones of different lengths covering the same region, for libraries having the same cloning enzyme.
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