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Studies employing this technique have recently determined that analysis of multiple clinical isolates is necessary due to the high rate of in vivo mutation [16], [21].
Because of these variations, more extensive studies involving multiple clinical isolates are needed in order to draw a definite conclusion about any trends in the pmpD gene transcription pattern in clinical isolates versus prototype strains.
We previously reported that unformulated RC-101 was active against multiple clinical isolates of HIV-1 from all subtypes, and was neither toxic nor proinflammatory to cervicovaginal cells or tissues both in vitro and ex vivo [7].
Our study demonstrated the utility of next-generation sequencing coupled to traditional microbiological testing method in a retrospective epidemiological study involving multiple clinical isolates.
Nucleotide blast search on NCBI limited to P. aeruginosa showed that the former can be found in multiple clinical isolates, while the latter is identical to RP73 only in strain 8380, isolated from the human gut.
In addition to those reported here, further tests of ATCC 36031 in comparison to other clinical isolates has led to the recommendation that multiple clinical isolates be utilized in disinfectant testing [ 67].
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Multiple serotype 14 clinical isolates were also genotyped by multilocus sequence typing (MLST).
Validation study also confirmed that the trimethoprim-gentamicin synergistic cocktail effectively inhibited the growths of multiple strains of uropathogenic clinical isolates.
However, if clinical isolates exhibit multiple evolutionary histories, pathogenicity would more likely reflect an adaptive advantage conferred by the acquisition of multiple virulence traits in different strains.
The genetic diversity among the clinical isolates suggests multiple origins of the genetic material required for ROS resistance in these strains.
An earlier study indicated that respiratory strains of Cpn clinical isolates contain multiple copies of the tyrP gene, whereas vascular strains contain a single copy [ 8].
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