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Multiple cleavage sites were identified for both falcipains (Fig. 2a d, top arrows).
In any event, our overall result is clear: falcipain-2 and falcipain-3 are capable of rapid hydrolysis of hemoglobin at multiple cleavage sites.
Sequencing of 5'RACE products revealed multiple cleavage sites within the predicted TMV-siRNA-interacting region in CPSF30 mRNA, with only one of them corresponded to the predicted canonical site for a cleavage directed by TMV-Cg-siR221, one of three viral siRNAs predicted to interact with the target (Fig 5C, upper panel).
Multiple cleavage sites that were associated to the same poly(A) signal were omitted.
Interestingly, we also identified multiple cleavage sites within 16S, 23S and 5S rRNA.
This result further suggests that PTEN protein sequence contains multiple cleavage sites.
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Reads mapped from the transcription start site (TSS) library showed high coverage of multiple sites, suggesting multiple 5′ cleavage sites and transcripts with modified 5′-phosphate groups (fig. 1 B).
Tryptic digests of fractions containing multiple bracelet cyclotides on the other hand resulted in unclear fragmentation patterns due to multiple enzyme cleavage sites and hence fragment-ion overlay.
Multiple aggregated cleavage sites occurring after a single poly(A) signal (only ~1% of total poly(A) sites in our procedure) were discarded.
No H5 or H7 AIVs contained multiple basic cleavage sites, a known molecular determinant for HPAI; therefore, all were classified as LPAI.
Granins contain multiple protease and peptidase cleavage sites and are named based on their biological activities, e.g. Catestatin, Vasostatin and Pancreastatin [ 20- 23].
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