The results showed that both Tau/Phi GSTs were located on multiple chromosomes with non-random distributions.
Each chromosome had between 2 and 19 genes, with several of the PCGs occurring on multiple chromosomes with evidence for modest nucleotide divergences between these different chromosome copies.
Most recently, Visscher reported a large-scale linkage study with 11,214 sibling pairs showing that additive genetic variance is spread across multiple chromosomes, with no evidence of large between-chromosome epistatic effects (Visscher et al. 2007).
In Spirochaetes, predictions using the profiles of Firmicutes, Chlamydiae and Proteobacteria profiles resulted in unique dif sequences in species with single chromosomes, and the profiles of Firmicutes were used for the predictions of 12 chromosomes in 6 species with multiple chromosomes, all with HMMER scores above the validation thresholds.
As previously shown in Proteobacteria [ 46], the XerC binding site is more variable and the XerD binding site is more conserved in all phyla, both for genomes with single chromosomes and for those with multiple chromosomes, presumably due to the interaction between XerD and FtsK for the initiation of first strand exchange [ 14].
Lastly, our previous study of bacterial genomes with multiple chromosomes included several Burkholderia genomes, which exhibited increased evolutionary rates consistent with reduced expression on secondary chromosomes (Cooper et al. 2010).
Profiles of aCGH showed no chromosomal abnormalities in the tumor from 2002, whereas the specimen from 2012 demonstrated multiple chromosome rearrangements with specific losses in large chromosomal regions in 5q, 8, 9q, 10, 12q, 13q, 14, 16q, 17, 18q, and 21 and a gain of 9p (Fig. 3).
The same prediction method was applied for 66 organisms with multiple chromosomes, totaling 142 chromosomes, where we could predict dif sequences in 63 organisms with 137 chromosomes (Table 1).
Among genomes with multiple chromosomes, IM and chromosome length were not correlated (Fig. 4).
30 bacteria with multiple chromosomes were removed as well.
It showed the potential to assemble more complex genomes with multiple chromosomes containing more repetitive sequences.
