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This would require multiple chromosomes to rely on their heterochromatin tethers to achieve coorientation (Hughes et al. 2009).
Consequently, the combination of fluorochrome and cytogenetic position allows multiple chromosomes to be identified in a single FISH reaction.
Clusters where the parental genes could have come from either an autosome or the Z were discarded, as were all clusters with retrogenes on multiple chromosomes to avoid biasing results toward autosome-to-autosome movement.
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There were about 2% of ETS, which could not be unambiguously assigned to the bovine genome sequence, because of matches to multiple chromosomes due to repetitive sequence motifs.
Surprisingly, some unannotated TARs were connected to read ends that mapped to multiple chromosomes, possibly due to mapping to repetitive sequences, erroneous mapping or possible cross-chromosome splicing (trans-splicing), a rare phenomenon that was previously observed [ 41].
Finally, the blast output was fed into custom Perl script to remove any transcripts that hit multiple chromosomes at different locations (chimeras) to generate novel Velvet-Oases transcripts.
In contrast, bacteria that have multiple chromosomes seem to segregate them by using independent, orthogonal machineries specific for each chromosome [ 19], and don't appear to have anything as general or as scalable as a mitotic spindle.
In contrast to the events seen in chromothripsis, the rearrangements seen in these prostate cancers affected multiple chromosomes at once.
The dispersal to multiple chromosomes may be related to the large segmental duplications in which these arrays are embedded.
Having validated the comparative genomics approach in the Vibrionaceae, we decided to extend the analysis to other phylogenetic groups that present genomes with multiple chromosomes in order to analyze the adaptation of a complex genetic network, like the SOS response, to such genomic environments.
It remains to be studied in further publications, whether bringing multiple chromosomes together leads to even stronger effects.
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