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Multiple chromosomal replication origins might have arisen by capture of viral or plasmidic replication origins and their respective associated initiator factor [21].
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The chromosomal replication origins (oriC) of gram positive, acid-fast actinomycetes have been investigated in streptomycetes and mycobacteria.
The recovery efficiency of two early chromosomal replication origins, ARS305 and ARS607, in the immunoprecipitated material relative to the input material was determined by real-time PCR.
Their loading and activation at chromosomal replication origins are the most important events in replication initiation.
Here, we applied a related approach to characterize chromosomal replication origins and termination sites in C. glabrata.
There are chromosomal replication origins that are only conserved in specific species, as illustrated by the conservation of oriC2-cdc6E in Haloarcula species.
In Caulobacter, the chromosomal replication origin is positioned at the cell pole, and upon initiation of replication, a copy of the origin sequence is moved to the opposite cell pole.
Their chromosomal replication origin has the typical organization for P. putida, which is distinct from the enteric-type origins (Yee & Smith, 1990; Weigel et al., 1997;).
This indicates that the W619 genome might have undergone a major DNA rearrangement that involved swapping two DNA segments that are flanking the chromosomal replication origin.
Chromosomes with multiple DNA replication origins are a hallmark of Eukaryotes and some Archaea.
Eukaryotic chromosomes are replicated from multiple replication origins.
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