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The increased solubility of heavily cholesteryl modified siRNA in the presence of rHSA facilitated duplex annealing and consequent interaction that allowed in vivo studies using multiple cholesteryl modifications.
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A structural-activity-based screen of in vitro EGFP-silencing was used to select optimal siRNA designs containing cholesteryl modifications within the sense strand that were used for in vivo studies.
In this work, we exploit the intrinsic transport properties of human serum albumin to tune the blood circulatory half-life, hepatic accumulation and gene silencing; based on the number of siRNA cholesteryl modifications.
Multiple genetic modifications were made to the genome.
Multiple surface modifications have been studied to prevent bacterial growth on implants.
We concurrently map relative levels of multiple histone modifications across multiple samples, each comprising as few as a thousand cells.
This variability originated from different histone variants as well as from diverse and multiple peptide modifications.
In vertebrates, Drp1 is subjected to multiple posttranslational modifications, including phosphorylation, ubiquitination and sumoylation [26].
Multiple genetic modifications in pigs can essentially benefit research on agriculture, human disease and xenotransplantation.
We propose that a combination of multiple histone modifications regulates the timing of replication origin firing.
The importance of multiple histone modifications in regulating transcription is well established.
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