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In the current process, multiple centrifugation steps with chemical de-emulsifiers are used for product recovery, posing economic and environmental burdens on the process.
This eliminates the need for purification via multiple centrifugation steps.
Previous studies have traditionally purified NIR-gold nanoparticle mixtures through multiple centrifugation steps and more recently utilized regenerated cellulose membranes (RCM) in a novel synthesis process named "DiaSynth" [22, 37].
Ultimately, the Cr-AuNp probes were purified using multiple centrifugation steps to remove free chromate in the suspension before the probes could be used for cellular studies.
To specifically address the issue of myocyte dedifferentiation and proliferation, we employed multiple centrifugation steps and Percoll gradient separation, resulting in highly-pure myocytes (Figure 1, and Figures S1 S2).
Other techniques for producing highly pure platelet samples often require multiple centrifugation steps.
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Ninety-four percent of the OVA AuNVs were recovered throughout the multiple centrifugation and washing steps with PBS.
A typical manufacturing process might employ a similar strategy (Lapinskas, 2010) with multiple centrifugation and resuspension steps needed to improve removal of soluble contaminants (e.g., cell metabolites, serum based proteins, and remaining growth factors).
The protocol previously described for DAPI TFI and image analysis consisted of multiple centrifugation and reagent addition steps, which was not amenable for ultrahigh-throughput screening.
Using this robot we demonstrate how centrifugation steps with multiple precipitations, most notably through a precipitation step of SDS in isopropanol, lead to high purity plasmid DNA and make possible high-throughput transfections into mammalian cells for functional gene annotations.
Cardiomyocytes isolated from rodent hearts were purified by multiple centrifugation and Percoll gradient separation steps, and the purity verified by immunostaining and RT-PCR.
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