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Fluorescence microscopic imaging is advantageous for high-content assays that assess in vivo drug effects using multiple cellular response parameters [7].
Alternatively, a compendium approach examining multiple cellular response parameters (e.g., gene expression levels and growth rates) can be used to infer the drug targets of a novel compound based on reference bioactivity profiles of well-characterized drugs [5], [6].
(Although the differences in LANA expression with and without rapamycin reached statistical significance, the variation was under 8%.) Thus, while mTOR can regulate multiple cellular response pathways, rapamycin appeared to have a disproportionate impact on RTA and its downstream lytic genes compared to cellular or latent viral proteins.
Overwhelming evidence suggested that the TP53 tumor suppressor gene is a central regulatory node of multiple cellular response pathways to endogenous or exogenous stresses [ 20].
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Stress-activated protein kinases regulate multiple cellular responses to a wide variety of intracellular and extracellular conditions.
PKC participates as a mediator of signal transduction during multiple cellular responses to cellular signals which stimulate cell proliferation, and differentiation.
Downstream from the MAPks are a range of transcription factors which control multiple cellular responses such as inflammation, proliferation, and apoptosis [14], [15], [16].
TWEAK (Tumor necrosis factor like WEAK inducer of apoptosis) is highly expressed by different immune cells and triggers multiple cellular responses, including control of angiogenesis.
Finally, the proposed method allows for parallel identification of genes relevant to multiple cellular responses, which makes it an efficient high-throughput analysis.
Cyclic AMP (cAMP) signaling plays an important role in regulating multiple cellular responses, such as growth, morphogenesis, and/or pathogenicity of eukaryotic organisms such as fungi.
First described as a weak apoptosis inducer, TWEAK triggers multiple cellular responses [9] ranging from proliferation to cell death, including control of angiogenesis [10], [11], [12].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com