Fluorescence microscopic imaging is advantageous for high-content assays that assess in vivo drug effects using multiple cellular response parameters [7].
Alternatively, a compendium approach examining multiple cellular response parameters (e.g., gene expression levels and growth rates) can be used to infer the drug targets of a novel compound based on reference bioactivity profiles of well-characterized drugs [5], [6].
In this study we characterized the tolerated and toxic profile of As2O3 exposure by analyzing multiple cellular survival parameters.
Although still limited by low target cell numbers and loss of a significant proportion of the starting material post fixation/permeabilization and washing steps, our protocol permits the simultaneous analysis of multiple cellular (HSC, MPP, GMP/CMP, MEP), and intracellular parameters in HSC/HPC, akin to studies of more mature hematopoietic cells [6].
Given that Ca2+ signals are a key regulator in multiple cellular functions, age-related changes in this parameter are relevant for the aging process [ 15].
MDVM is an emerging new technology that permits single living cells to be labeled with multiple probes whose fluorescence is responsive to specific cellular parameters of interest.
The combination of multiple fluorescence detection parameters for improved fluorophore discrimination promises to substantially enhance our ability to visualize multiple cellular targets with sub-diffraction-limit resolution.
Maintenance of plasma osmolality and sodium concentrations within tight limits is one of the most highly regulated parameters of body physiology [35]; osmotically induced variations in cell volume can exert adverse effects on multiple cellular functions [36].
The resulting parameter values were then validated by determining how well independent experimental datasets were reproduced for multiple cellular functions.
