Sentence examples for multiple cell passages from inspiring English sources
To validate this single nucleotide change, each E006AA cDNA PCR product, generated from multiple cell passages, was sequenced using both sense and anti-sense primers.
However, such a difference could not be detected after multiple cell passages.
Stable integration of the transgene allows for the testing of target gene expression over an extended period of time and after multiple cell passages.
JEV-VLP production was stable after multiple cell passages and 100% cell expression was maintained without detectable cell fusion or apoptosis.
The DNA methylation patterns include anomalies in key genes implicated in the pathogenesis of RA and are stable for multiple cell passages.
8 Changes in expression of these genes were maintained throughout multiple cell passages for both leiomyoma and myometrial cell cultures (data not shown).
In contrast with v-Src-transformed cells, TGF-β3 mRNA is up-regulated in QNR/v-srcts/ICN cells, once ICN stable expression is established, and is maintained through multiple cell passaging despite the presence of an active oncoprotein.
Therefore, the diabetic epigenotype that we have identified represents a hyperglycemia-associated DNA methylation pattern that is persistent despite multiple cell culture passages under normoglycemic conditions.
Close examination of whole genome sequence of 54 SARS-CoV isolates identified before 14th October 2003, including 22 from patients in Singapore, revealed the mutations engendered during human-to-Vero and Vero-to-human transmission as well as in multiple Vero cell passages in order to refine our analysis of human-to-human transmission.
Importantly, expression of CD133 by FACS has been tested at different time points throughout several years, in multiple conditions of cell passages and confluence.The relatively low variability on CD133 expression across time and conditions suggests a dynamic balance between CD133-expressing and non-expressing fractions in both cell lines.
Each individual has components in their blood from which can be formed a mechanically stable hydrogel having the capacity to maintain cellular phenotype and support cellular proliferation of multiple cell types through several culture passages ex vivo.
