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While nacre has demonstrated its effectiveness in multiple cell culture and animal models, it remains a relatively underexplored biomaterial.
Although multiple cell culture models demonstrated oncogenic potential of several HCV proteins, most transgenic mouse models expressing these proteins did not develop HCC [2].
Multiple cell culture models have demonstrated the oncogenic potential of several HCV proteins; however, most transgenic mouse models expressing HCV proteins did not develop HCC [2].
The present manuscript fills this gap of knowledge and shows for the first time that capsaicin can potently inhibit the proliferation of human SCLCs using multiple cell culture and in vivo models.
Unlike previous studies that compare multiple cell culture conditions for one hESC line, or one differentiation protocol for multiple hESC lines, our approach was to directly compare multiple hESC lines under several culture and differentiation conditions [4], [31] [34].
To compare multiple cell culture conditions within a given experiment, the mean and standard error (SEM) across experimental groups were analyzed by One-Way Analysis of Variance (ANOVA) followed by Student-Newman-Keuls (SNK) post hoc test except when data were not normally distributed when ANOVA on Ranks and Mann-Whitney-U analyses were performed.
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For example, it may be possible to take advantage of advances in fluorescent cell bar-coding to enable immunophenotyping of multiple cell cultures simultaneously in one experiment [50].
Our approach of using multiple cell cultures and cytotoxicity end points has proven valuable in revealing the combinatorial nanoparticle-mediated effects important for hazard assessment.
In vitro tests performed on cell lines confirm the effectiveness of the device as an experimental tool for measurements in dynamic and multiple-cell cultures and cocultures.
The proof-of-concept for targeting Mdmx has been successfully demonstrated in multiple cultured cell culture models and, importantly, in an in vivo mouse model, which demonstrated tolerance of mouse tissues to Mdmx deletion.
Thereby gene expression in a sample under investigation is reported as a multiple of cell culture expression.
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