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Sigmoidal kinetics can arise from many possibilities, such as the oligomeric state of the enzyme, multiple catalytic sites, or slow conformational changes.
The data conformed to a kinetic mechanism where the first site to fill is active but when both sites are filled, distinct kinetic parameters are observed according to the expression first named by W. W. Cleland as a "2/1" inhibitor function and later adapted to cooperative multiple catalytic sites in the form v = kcat2((A + kcat1 Km2 A/ kcat2))/(A + Km2 A + Km1 Km1).
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The results showed that these catalysts contained multiple types of catalytic sites and that the types of sites were a strong function of the support pore size.
Because of their large catalytic sites, multiple chemicals have been shown to inhibit carboxylesterases.
While FAS-I is a single, large polypeptide chain folded into multiple domains that harbor the catalytic sites required for the biosynthesis of fatty acids, the FAS-II is composed of a series of discrete soluble enzymes that act successively and repetitively to elongate the fatty acid chains produced by FAS-I (Sylvain Cantaloube et al., 2011).
Based on the multiple sequence analysis and the architecture of catalytic sites, aaRSs are divided into two classes of ten aaRSs each [ 13].
Again, staging oxygen by multiple injection schemes increases attainable yields but requires larger reactor volume and catalytic sites with low reaction order (⪡ 1) in oxygen.
Activation of surfaces may be classified as follows; (i) originally active surfaces, (ii) intermediates produced by reaction with surface atoms, (iii) formation of active sites with functional groups, (iv) formation of active surface compounds, (v) cooperation of multiple catalytic processes.
Whether such selective proteasomal inhibition in the context of multiple myeloma makes it more potent and less toxic than NPI-0052, which appears to target all three catalytic sites, remains unknown.
Catalytic sites alone cannot overcome these constraints.
Rates of catalytic reactions are controlled by substrate concentrations at catalytic sites.
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